DL-Valine methyl ester hydrochloride is primarily used in organic synthesis and pharmaceutical research. It serves as a key intermediate in the production of peptides and amino acid derivatives, which are essential in developing various therapeutic agents. This compound is particularly valuable in the synthesis of chiral molecules due to its enantiomeric form, enabling the creation of optically active compounds. Additionally, it is utilized in the preparation of prodrugs, enhancing the bioavailability and stability of active pharmaceutical ingredients. Its role in biochemical studies also aids in understanding enzyme mechanisms and protein interactions.