Nalpha-(2,4-Dinitrophenyl)-L-arginine is primarily used in biochemical research as a reagent for studying enzyme activity, particularly in the context of arginine-specific enzymes. It serves as a substrate or inhibitor in assays to investigate the function and kinetics of enzymes like arginase or nitric oxide synthase. The compound’s dinitrophenyl group allows for easy detection and quantification, making it valuable in spectrophotometric or chromatographic analyses. Additionally, it is utilized in the development of enzyme inhibitors for therapeutic applications, particularly in conditions where arginine metabolism plays a critical role, such as cardiovascular diseases or immune regulation. Its specificity and reactivity make it a useful tool in both academic and pharmaceutical research settings.