N-Acetyl-DL-alanine is widely used in biochemical research as a building block for peptide synthesis. It serves as a precursor in the production of various peptides and proteins, aiding in the study of their structure and function. Additionally, it is utilized in the development of pharmaceutical compounds, particularly in the synthesis of drugs that target specific enzymes or receptors. Its role in metabolic studies is also significant, as it helps in understanding amino acid metabolism and related pathways.