Ethyl 4-amino-4-oxobutanoate is primarily used in organic synthesis as a versatile intermediate for the production of various pharmaceuticals and bioactive compounds. It plays a key role in the synthesis of amino acid derivatives and peptides, which are essential in drug development. Additionally, it is utilized in the preparation of heterocyclic compounds, which are important in the creation of agrochemicals and medicinal agents. Its application extends to research laboratories, where it serves as a building block for designing and testing new chemical entities with potential therapeutic properties.