Used primarily as a photoactivatable amino acid analog in biochemical and proteomic research. Enables covalent crosslinking of interacting proteins upon UV irradiation due to the reactive diazirine group, which generates highly reactive carbenes. The Fmoc group allows for solid-phase peptide synthesis, making it suitable for incorporation into peptides. Commonly applied in photoaffinity labeling studies to identify and characterize transient or weak protein-ligand and protein-protein interactions. Its stereochemistry supports natural peptide backbone compatibility, enhancing its utility in studying biologically relevant systems.