Widely used in peptide synthesis as a protected chiral building block, this compound enables the incorporation of constrained cyclobutane amino acid derivatives into peptide chains. The Fmoc group allows for mild base-labile protection, compatible with solid-phase synthesis, making it valuable in the preparation of structurally modified peptides and peptidomimetics. Its rigid cyclobutane backbone influences peptide conformation, enhancing stability and selectivity in biological applications. Commonly employed in drug discovery for developing enzyme inhibitors and receptor ligands where backbone rigidity improves metabolic stability and binding affinity.