Used as a protected intermediate in peptide synthesis, particularly in the preparation of complex peptides and proteins where selective protection of the histidine side chain is required. The tert-butoxycarbonyl (Boc) groups prevent unwanted reactions at the imidazole ring during coupling steps, ensuring higher yield and purity. It is especially valuable in solid-phase peptide synthesis (SPPS) and in the production of pharmaceutical-grade peptides. Its stability under basic and mildly acidic conditions allows for stepwise assembly of peptide chains before final deprotection.