Widely used in enzymatic assays, particularly in protease activity studies. It serves as a fluorogenic substrate, releasing 7-amino-4-methylcoumarin (AMC), a highly fluorescent compound, upon cleavage by specific enzymes such as aminopeptidases. This property makes it valuable in high-throughput screening for drug discovery and in monitoring enzyme kinetics. Commonly employed in clinical diagnostics to assess enzyme levels in biological samples. Its sensitivity allows detection of low enzyme concentrations, enhancing accuracy in research and diagnostic applications.