Used in the synthesis of fluorescently labeled peptides for biochemical and biophysical studies. The EDANS moiety serves as a fluorescent donor in Förster resonance energy transfer (FRET) assays, making this compound valuable for monitoring enzyme activity, protein-protein interactions, and conformational changes in real time. Commonly employed in protease assays where cleavage of a peptide substrate leads to a measurable increase in fluorescence. The Fmoc group allows for standard solid-phase peptide synthesis, enabling easy incorporation into custom peptide sequences. Ideal for creating sensitive probes in high-throughput screening and diagnostic applications.