Widely used in fluorescence-based biochemical assays, this compound enables real-time tracking of cellular uptake and intracellular localization of peptides and proteins. Its primary application lies in labeling biomolecules for fluorescence microscopy, flow cytometry, and high-throughput screening. The FAM fluorophore provides strong green fluorescence, allowing sensitive detection in live or fixed cells. It is especially valuable in studying peptide-protein interactions, receptor binding, and drug delivery systems when incorporated into synthetic peptides via solid-phase peptide synthesis. The Fmoc group ensures compatibility with standard peptide coupling protocols, making it ideal for creating fluorescently tagged therapeutic or diagnostic peptides.