Used in fluorescence-based studies of peptide structure and dynamics, this compound serves as a key building block in solid-phase peptide synthesis where the EDANS fluorophore acts as a fluorescent donor in FRET (Förster resonance energy transfer) assays. Its incorporation into peptide sequences allows real-time monitoring of enzymatic activity, such as protease cleavage, due to the change in fluorescence upon separation from a quenching moiety. The Fmoc group enables stepwise synthesis by providing temporary N-terminal protection, while the tert-butyl ester protects the glutamic acid side chain, ensuring selective deprotection and minimal side reactions. Commonly applied in developing biosensors, diagnostic probes, and studying protein-protein interactions.