Used in fluorescence-based studies of enzyme activity, particularly for proteases. The compound incorporates EDANS, a fluorescent reporter group, allowing detection of cleavage events when paired with a quenching group. Commonly applied in the synthesis of peptide substrates where real-time monitoring of enzymatic reactions is required. Ideal for assays involving metalloproteases or other hydrolases due to the protected carboxylic acid group, which enhances stability during solid-phase peptide synthesis. Enables construction of sensitive diagnostic probes and high-throughput screening tools in drug discovery.