DL-3-Aminoisobutyric acid is used in biochemical and metabolic research as a marker for muscle protein turnover and DNA demethylation processes. It serves as a stable endogenous analog in studies related to nucleic acid metabolism and oxidative stress. Due to its structural similarity to amino acids involved in one-carbon metabolism, it is employed in assays investigating epigenetic regulation and methyl group transfer. Additionally, it has been utilized in tracer studies to monitor metabolic flux in mammalian systems, particularly in relation to thymine and pyrimidine degradation pathways. Its presence in urine is also monitored as a non-invasive biomarker in clinical and toxicological assessments.