(S)-2-Amino-3-chloropropanoic acid hydrochloride is primarily used in biochemical research and pharmaceutical development. It serves as a chiral building block in the synthesis of various biologically active compounds, particularly in the creation of peptide-based drugs. Its stereochemistry makes it valuable for studying enzyme interactions and designing inhibitors or substrates for specific enzymes. Additionally, it is utilized in the development of prodrugs and as an intermediate in the production of therapeutic agents targeting neurological and metabolic disorders. Its role in asymmetric synthesis also contributes to the advancement of medicinal chemistry and drug discovery.