Cis-4-Hydroxy-D-proline hydrochloride is widely used in biochemical research, particularly in the study of collagen and protein structures. It serves as a key component in the synthesis of peptides and modified amino acids, aiding in the exploration of protein folding and stability. This compound is also utilized in the development of pharmaceutical agents, especially those targeting collagen-related disorders. Additionally, it plays a role in the production of chiral catalysts and in asymmetric synthesis, contributing to advancements in organic chemistry and drug development. Its applications extend to the field of tissue engineering, where it is used to mimic collagen-like structures for biomaterial development.