L-2,4-Diaminobutyric acid hydrochloride is primarily used in biochemical research and pharmaceutical development. It serves as a precursor or intermediate in the synthesis of various biologically active compounds, including peptides and amino acid derivatives. Its role in studying metabolic pathways, particularly those involving amino acid metabolism, is significant. Additionally, it is utilized in the development of drugs targeting specific enzymes or receptors due to its structural similarity to naturally occurring amino acids. In some cases, it is also employed in the preparation of chiral catalysts for asymmetric synthesis, enhancing the efficiency and selectivity of chemical reactions.