Used in biochemical research as a non-natural amino acid to study enzyme mechanisms and protein interactions. It serves as a precursor in the synthesis of various peptides and proteins, aiding in the exploration of structural and functional properties. This compound is also utilized in the development of enzyme inhibitors, providing insights into metabolic pathways and potential therapeutic targets. Its nitro group makes it a useful probe in spectroscopic studies, helping to analyze protein dynamics and conformational changes. Additionally, it plays a role in the investigation of amino acid transport systems in cells.