Methyl 2-amino-4-methylpentanoate hydrochloride is primarily used in organic synthesis and pharmaceutical research. It serves as a key intermediate in the production of various bioactive compounds, including peptides and amino acid derivatives. Its application extends to the development of drugs targeting neurological disorders, as it can be utilized to modify or enhance the properties of therapeutic molecules. Additionally, it is employed in the study of metabolic pathways and enzyme interactions, aiding in the understanding of biochemical processes. Its stability and reactivity make it a valuable component in laboratory settings for synthesizing complex organic molecules.