L-beta-Homohydroxyproline hydrochloride is primarily used in biochemical research and pharmaceutical development. It serves as a building block in the synthesis of peptides and modified amino acids, which are essential for studying protein structures and functions. This compound is particularly valuable in the design of collagen-like peptides due to its structural similarity to hydroxyproline, a key component of collagen. Additionally, it is utilized in the development of drugs targeting collagen-related disorders, such as fibrosis and certain types of cancer. Its role in enhancing peptide stability and bioactivity makes it a critical tool in medicinal chemistry and drug discovery.