N-(tert-Butyl)-2-nitrobenzenesulfonamide is primarily used in organic synthesis as a protecting group for amines. Its application is significant in the preparation of complex molecules, particularly in peptide synthesis, where it helps to temporarily shield amine groups during reactions to prevent unwanted side reactions. The compound is also employed in the development of pharmaceuticals, allowing for selective modification of molecular structures. Its stability under various reaction conditions makes it a valuable tool in multi-step synthetic processes. Additionally, it finds use in the synthesis of agrochemicals and other fine chemicals, where precise control over functional groups is essential.