This chemical is primarily utilized in organic synthesis as a protective group for amines. It is often employed in peptide synthesis to safeguard the amine functionality during reactions, ensuring selective modification of other functional groups. The nitrobenzyl group can be removed under mild conditions, making it suitable for sensitive applications. Additionally, it serves as an intermediate in the preparation of pharmaceuticals and agrochemicals, where controlled release or protection of amine groups is critical. Its stability and ease of deprotection make it a valuable tool in complex synthetic pathways.