3,4-Dimethoxyphenylacetylene is primarily used as a building block in organic synthesis, particularly in the production of complex molecules. It serves as a key intermediate in the development of pharmaceuticals, where it is utilized to create compounds with potential therapeutic effects. Additionally, it is employed in the synthesis of agrochemicals, contributing to the development of pesticides and herbicides. Its structure allows for versatile chemical reactions, making it valuable in the creation of fine chemicals and specialty materials. In research, it is often used in the study of catalytic processes and the development of novel organic compounds.