This chemical is primarily used in bioconjugation and click chemistry applications. It serves as a reactive ester for coupling with amines, enabling the attachment of functional groups or labels to biomolecules like proteins, peptides, or antibodies. The alkyne group in its structure allows it to participate in copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions, a widely used method for linking molecules in a highly specific manner. Its polyethylene glycol (PEG) spacer enhances solubility and reduces steric hindrance, making it suitable for modifying biomolecules in aqueous environments. This compound is particularly valuable in drug delivery systems, diagnostic assays, and the development of bioconjugated probes for research purposes.