Used primarily as a crosslinking agent in bioconjugation, this compound enables the stable attachment of functional molecules such as peptides, proteins, or fluorescent labels to primary amines on biomolecules. Its N-hydroxysuccinimide (NHS) carbonate group reacts efficiently with amine groups under mild aqueous conditions, forming stable carbamate bonds. The strained alkyne in the bicyclic structure allows for subsequent bioorthogonal reactions, particularly copper-free click chemistry, making it ideal for labeling biomolecules in live cells or sensitive biological systems. Commonly employed in the development of antibody-drug conjugates (ADCs), diagnostic probes, and targeted therapeutics where controlled, two-step conjugation is required.