Cyanine7 DBCO is widely used in bioorthogonal chemistry for labeling and imaging biomolecules in live cells and tissues. Its near-infrared fluorescence allows deep tissue imaging with minimal background autofluorescence, making it ideal for in vivo imaging applications. The DBCO group enables copper-free click chemistry, which reacts rapidly and selectively with azide-functionalized molecules without toxic catalysts. This feature is especially valuable for labeling sensitive biological systems such as cell surface proteins, nucleic acids, and glycans. It is commonly employed in fluorescence microscopy, flow cytometry, and molecular imaging studies where high sensitivity and low interference are critical. Additionally, Cyanine7 DBCO is used in targeted imaging probes and drug conjugates for monitoring disease progression and therapeutic delivery in preclinical research.