Widely used in bioorthogonal chemistry for labeling biomolecules in live cells without interfering with natural biological processes. Its DBCO group reacts selectively with azides through copper-free click chemistry, making it ideal for imaging applications where copper toxicity is a concern. Commonly employed in fluorescence microscopy to track proteins, glycans, and nucleic acids in real time. The Cyanine3 fluorophore provides strong red fluorescence, enabling high sensitivity detection in cellular and molecular imaging. Also utilized in flow cytometry and in vivo imaging due to its stability and bright signal. Suitable for labeling antibodies, peptides, and oligonucleotides in diagnostic assays and research tools.