Used in bioconjugation and labeling applications, particularly in click chemistry reactions. The BCN group reacts efficiently with azides via copper-free click chemistry, making it ideal for labeling biomolecules in live cells without cytotoxic effects. PEG3 spacer enhances solubility and reduces nonspecific binding, improving the accessibility of biotin to streptavidin or avidin detection systems. Commonly used in proteomics, cellular imaging, and target identification workflows where sensitive detection and high specificity are required. Suitable for in vitro and in vivo applications due to its stability and biocompatibility.