Used in bioorthogonal labeling and detection applications, particularly in fluorescence-based imaging. Enables selective tagging of biomolecules in live cells through click chemistry, due to the presence of the alkyne group that reacts with azide-functionalized probes. Exhibits strong fluorescence with high photostability, making it suitable for confocal microscopy and flow cytometry. Commonly applied in studying cellular processes such as protein trafficking, membrane dynamics, and nucleic acid modifications without interfering with native biological functions.