Used in fluorescence detection techniques, particularly in signal amplification methods like tyramide signal amplification (TSA). It enables highly sensitive detection of low-abundance targets in immunohistochemistry, immunofluorescence, and in situ hybridization. The alkyne group allows for subsequent click chemistry reactions, facilitating the attachment of fluorescent dyes or other reporter molecules. This makes it valuable in multiplex labeling and imaging applications where precise localization and high sensitivity are required. Commonly used in research settings for cellular and tissue imaging, especially when co-localization studies or low-expression proteins need visualization.