Used in bioconjugation and click chemistry applications, particularly in labeling biomolecules such as proteins and nucleic acids. Its strained alkyne group enables copper-free azide-alkyne cycloaddition, making it suitable for live-cell imaging and in vivo studies where copper toxicity is a concern. The carboxylic acid functionality allows for easy amide coupling to amines, facilitating the attachment to various biomolecules or solid supports. Commonly employed in pharmaceutical research and diagnostic probe development due to its fast reaction kinetics and bioorthogonality.