Used as a key reagent in click chemistry, particularly in copper-free azide-alkyne cycloaddition reactions. Its strained cyclooctyne core enables rapid and selective bioconjugation with azide-containing biomolecules, making it valuable for labeling proteins, nucleic acids, and live cells without toxic catalysts. Commonly employed in bioimaging, drug delivery systems, and the development of diagnostic probes due to its fast reaction kinetics and biocompatibility. Also utilized in material science for surface functionalization and polymer modification.