Primarily utilized in bioconjugation and biochemical research, Biotin-PEG2-C4-alkyne serves as a versatile linker for attaching biomolecules to various surfaces or probes. The biotin moiety enables high-affinity binding to streptavidin or avidin, facilitating specific labeling and immobilization. Its alkyne group enables click chemistry reactions, particularly with azide-containing molecules, facilitating the creation of stable triazole linkages. This makes it valuable in the development of targeted drug delivery systems, where precise attachment of therapeutic agents to carriers is crucial. Additionally, it is employed in the study of protein interactions and cellular processes, allowing researchers to label and track biomolecules with high specificity. Its PEG spacer enhances solubility and reduces steric hindrance, improving the efficiency of conjugation reactions in aqueous environments.