Used primarily in bioimaging and bioconjugation, it serves as a fluorescent probe for labeling biomolecules in cellular studies. Its alkyne group enables click chemistry reactions, facilitating the attachment to azide-modified targets. The PEG4 spacer enhances solubility and reduces steric hindrance, improving binding efficiency. It is widely applied in live-cell imaging, protein tracking, and diagnostic assays due to its bright fluorescence and compatibility with biological systems.