This chemical is widely used in bioconjugation and click chemistry applications due to its reactive DBCO (dibenzocyclooctyne) group, which enables efficient and selective coupling with azide-containing molecules. The PEG4 spacer enhances solubility and flexibility, making it suitable for linking biomolecules in aqueous environments. The Boc (tert-butyloxycarbonyl) protecting group allows for controlled deprotection and subsequent functionalization, making it valuable in peptide synthesis and drug delivery systems. Its applications include labeling proteins, antibodies, and other biomolecules for imaging, diagnostics, and targeted therapy research.