This compound is widely used in bioconjugation and click chemistry applications due to its DBCO (dibenzocyclooctyne) group, which reacts efficiently with azides without the need for a catalyst. The PEG4 spacer enhances solubility and provides flexibility, making it suitable for linking biomolecules such as proteins, peptides, or antibodies. The carboxylic acid group allows for further conjugation to amines or other functional groups, enabling the creation of stable and specific bioconjugates. It is particularly valuable in drug delivery systems, diagnostic imaging, and the development of targeted therapies, where precise and efficient molecular labeling is essential.