Used primarily in research as a potent alkylating agent, this chemical is known for its ability to induce mutations and carcinogenesis in experimental models. It is commonly applied in studies related to cancer development, DNA repair mechanisms, and mutagenicity testing. Due to its high reactivity, it modifies DNA by transferring methyl groups, leading to base pair changes that help scientists understand genetic instability and tumor formation. It is also used in the preparation of other chemical compounds where selective alkylation is required. Handling requires extreme care because of its high toxicity and carcinogenic potential.