Used primarily in research settings as a potent alkylating agent and carcinogenic compound, this chemical serves as a model substance for studying nitrosamine-induced DNA damage and mutagenesis. It is employed in toxicology studies to evaluate cellular repair mechanisms and the biological impact of DNA alkylation. Due to its high reactivity and toxicity, it is not used in industrial or commercial applications but remains relevant in laboratory investigations of chemical carcinogenesis and cancer pathway modeling.