Used in targeted cancer therapies as a potent DNA-alkylating agent, particularly in antibody-drug conjugates (ADCs). Its high cytotoxicity enables selective destruction of tumor cells when delivered via monoclonal antibodies that bind to cancer-specific antigens. The compound’s structure allows for controlled activation in the tumor microenvironment, minimizing damage to healthy tissues. It is especially effective in solid tumors with high antigen expression. Stability from the Boc and MOM protecting groups facilitates efficient conjugation and prolonged circulation before activation.