Trolline is primarily used in scientific research, particularly in the field of natural products chemistry and pharmacology. It serves as a reference compound for studying the biological activities of alkaloids, especially those with complex polycyclic structures. Due to its structural uniqueness, it is also utilized in synthetic studies aimed at developing new methodologies for constructing intricate nitrogen-containing ring systems. Its presence in certain plant extracts has prompted investigations into potential neuroactive or enzyme-inhibiting properties, although practical therapeutic applications remain under exploration. Trolline is not currently used in commercial pharmaceuticals or industrial applications.