Trachelanthine is primarily used in scientific research, particularly in the study of alkaloid biosynthesis and structure-activity relationships in natural products. It serves as a reference compound in phytochemical investigations due to its presence in certain medicinal plants. Its unique molecular framework also makes it a candidate for evaluating biological activity, including potential neuroactive and antimicrobial properties. However, it is not currently used in commercial pharmaceuticals or industrial applications, and its main role remains confined to laboratory-based research in organic and medicinal chemistry.