AT7867 dihydrochloride is primarily utilized in biochemical and pharmacological research due to its role as a potent inhibitor of protein kinases, particularly AKT and p70 S6K. It is widely employed in studies focused on understanding cellular signaling pathways, especially those involved in cell growth, survival, and proliferation. Researchers use it to investigate the effects of inhibiting these pathways in various cancer models, as AKT and p70 S6K are often implicated in tumor development and progression. Additionally, it serves as a valuable tool in exploring potential therapeutic strategies for diseases where these kinases play a critical role. Its application extends to in vitro and in vivo experiments, helping to elucidate mechanisms of action and evaluate efficacy in preclinical settings.