Akt1 and Akt2-IN-1 is primarily used in research settings to study the role of Akt signaling pathways in various biological processes. It is a potent inhibitor of Akt1 and Akt2 kinases, which are involved in cell survival, growth, and metabolism. Researchers utilize this compound to investigate its effects on cancer cell proliferation, as Akt signaling is often dysregulated in many types of cancers. By inhibiting these kinases, it helps in understanding the mechanisms of tumor growth and resistance to therapies. Additionally, it is used in preclinical studies to explore potential therapeutic applications for diseases where Akt pathways play a critical role, such as diabetes and neurodegenerative disorders. Its application extends to studying cellular responses to stress and apoptosis, providing insights into novel treatment strategies.