A-674563 is primarily utilized in research settings as a potent and selective inhibitor of Akt, a serine/threonine kinase involved in various cellular processes. Its application is significant in studying the role of the Akt signaling pathway in cancer biology, as this pathway is often dysregulated in many types of cancer. Researchers use A-674563 to investigate potential therapeutic strategies targeting Akt to inhibit tumor growth and survival. Additionally, it is employed in preclinical studies to understand the molecular mechanisms underlying cell proliferation, apoptosis, and metabolism, providing insights into the development of targeted cancer therapies.