Pim1/AKK1-IN-1 is primarily used in research settings to study its inhibitory effects on Pim-1 kinase and AKK1 kinase, which are involved in various cellular processes such as cell survival, proliferation, and metabolism. Its application is significant in cancer research, where it helps in understanding the role of these kinases in tumor growth and progression. By inhibiting these kinases, researchers can explore potential therapeutic strategies for cancers that are driven by their overexpression or hyperactivity. Additionally, it serves as a valuable tool in biochemical assays to investigate signaling pathways and develop targeted therapies for diseases associated with dysregulated kinase activity.