Cibacron Blue 3G-A is widely used in biochemistry and molecular biology for affinity chromatography. It is particularly effective in purifying proteins and enzymes, such as dehydrogenases, kinases, and albumin, due to its ability to bind specifically to nucleotide-binding sites. This dye is also utilized in the separation and purification of biomolecules like antibodies and nucleic acids. Additionally, it serves as a tool in studying protein-ligand interactions and enzyme kinetics. Its application extends to diagnostic assays and research, where it aids in identifying and isolating specific biological molecules.