Used in affinity chromatography for the purification of proteins, especially in immobilized metal ion affinity chromatography (IMAC). The epoxy-activated Sepharose matrix binds iminodiacetic acid, which then chelates divalent metal ions like nickel, cobalt, or copper. These metal ions interact with histidine-tagged proteins, allowing selective capture and purification from complex mixtures. Commonly applied in recombinant protein isolation, this resin offers high binding capacity, good stability, and reusability. Ideal for both laboratory-scale and industrial protein purification processes.