Used in affinity chromatography for the purification of proteins and peptides. The epoxy-activated Sepharose matrix binds iminodiacetic acid, which then chelates metal ions like nickel or cobalt. This allows selective capture of histidine-tagged recombinant proteins from complex mixtures. After binding, target proteins are eluted under mild conditions, preserving activity. The resin is stable, reusable, and suitable for both laboratory-scale and industrial applications in biotechnology and pharmaceutical research.