EHNA hydrochloride is primarily used in biochemical research as a potent and selective inhibitor of adenosine deaminase (ADA). This enzyme plays a crucial role in purine metabolism, and its inhibition is studied to understand various physiological and pathological processes. Researchers utilize EHNA hydrochloride to investigate the role of adenosine in immune regulation, inflammation, and cancer biology. It is also employed in studies focusing on the therapeutic potential of ADA inhibition in diseases such as leukemia and other hematological disorders. Additionally, EHNA hydrochloride aids in exploring the effects of adenosine accumulation in cardiovascular and neurological systems, providing insights into potential treatments for conditions like ischemia and epilepsy. Its application extends to drug development, where it serves as a tool to evaluate the efficacy of new ADA-targeting compounds.