Used in bioconjugation and pharmaceutical research as a bifunctional linker, particularly in the development of antibody-drug conjugates (ADCs) and targeted therapeutics. Its tetrazine group enables rapid, bioorthogonal ligation via inverse electron-demand Diels-Alder (IEDDA) reactions, allowing efficient and selective coupling to trans-cyclooctene (TCO)-modified biomolecules. The extended oligoethylene glycol chain enhances water solubility and provides flexibility, improving binding kinetics and reducing steric hindrance. The terminal amine group (as hydrochloride salt) facilitates covalent attachment to carboxylated compounds through standard coupling reagents. This compound is especially valuable in pretargeting strategies, imaging probes, and the construction of complex molecular architectures where fast, specific, and catalyst-free conjugation is required.