Palmitoyl coenzyme A lithium salt is widely used in biochemical research to study lipid metabolism and enzyme kinetics. It serves as a substrate for enzymes like acyl-CoA synthetases and acyltransferases, helping to understand fatty acid oxidation and biosynthesis pathways. This compound is also employed in assays to measure the activity of carnitine palmitoyltransferase, a key enzyme in mitochondrial fatty acid transport. Additionally, it is utilized in the investigation of lipid signaling pathways and the role of fatty acyl-CoAs in cellular processes. Its application extends to studying metabolic disorders, such as obesity and diabetes, by providing insights into lipid-related dysfunctions.